OBTAINING A CLONA OF «NEETHLING-RIBSP/С7 ATTENUATED LUMPY SCIN DISEASE VIRUS
Keywords:
virus, clone, lumpy skin disease, harmlessness, vaccination, cattle.Abstract
The aim of this study was to obtain a harmless clone of the attenuated strain «NeethlingRIBSP» lumpy skin disease virus. To achieve this goal, the attenuated strain «Neethling-RIBSP» was
cloned by the method of limiting dilutions. In the subsequent selection of clones used the reaction of
plaque formation. As a result of cloning, 9 clones of the «Neethling-RIBSP» strain of Lumpy Dermatitis
Virus were obtained. Growth characteristics of clones of the «Neethling-RIBSP» strain of lumpy skin
disease virus were determined by infecting the cells of the culture of the testicular lambs (TU).
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Studies have shown that out of 9 clones obtained, only 4 had pronounced cultural properties and
accumulated in TU cell culture in titers of 5.5-6.0 lg TCID50/cm3. The remaining 5 clones replicated
poorly in TU cell culture. To determine the least areactogenic clone of the attenuated NeethlingRIBSP strain, animals were vaccinated with each clone intradermally in dilutions from 10-1 to 10-6
and 5 animals subcutaneously in a field dose. Studies have shown that both with subcutaneous and
intradermal administration of the Neethling-RIBSP/C7 clone, the experimental animals remained
clinically healthy, the body temperature of the experimental animals was within the physiological
norm, and no local reactions were noted at the injection site.
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